Composite

Part:BBa_K4803118:Design

Designed by: Kotaro MURAI   Group: iGEM23_UTokyo   (2023-10-10)


STAT3 responsive element-mini promoter-ZsGreen


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 133
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 171
    Illegal BamHI site found at 157
    Illegal XhoI site found at 167
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Major restriction enzyme recognition sequences (EcoRI XbaI SpeI PstI NotI BsaⅠ SapⅠ) are removed from this part.

Source

This biobrick is created by combining Part:BBa_K4803007(STAT3 responsive element), Part:BBa_K4803010(mini promoter), BBa_K3190204(ZsGreen).

References

[1] Wang, Y., Boxel-dezaire, A. V., Cheon, H., & Stark, G. R. (2013). STAT3 Activation in Response to IL-6 Is Prolonged by the Binding of IL-6 Receptor to EGF Receptor. BIOLOGICAL SCIENCES, 110(42), 16975–16980. https://doi.org/https://doi.org/10.1073/pnas.1315862110

[2] Daringer, N. M., Dudek, R. M., Schwarz, K. A., & Leonard, J. N. (2014). Modular extracellular sensor architecture for engineering mammalian cell-based devices. ACS synthetic biology, 3(12), 892-902. https://doi.org/10.1021/sb400128g

[3] Xu, J., Lin, H., Wu, G., Zhu, M., & Li, M. (2021). IL-6/STAT3 is a promising therapeutic target for hepatocellular carcinoma. Frontiers in oncology, 11, 760971. https://doi.org/10.3389/fonc.2021.760971

[4]Wei, W., Tweardy, D. J., Zhang, M., Zhang, X., Landua, J., Petrovic, I., Bu, W., Roarty, K., Hilsenbeck, S. G., Rosen, J. M., & Lewis, M. T. (2014). STAT3 signaling is activated preferentially in Tumor‐Initiating cells in Claudin‐Low models of human breast cancer. Stem Cells (Dayton, Ohio), 32(10), 2571-2582. https://doi.org/10.1002/stem.1752